关岭牛MyoDI基因启动子上转录因子结合位点的筛选

doi:10.11843/j.issn.0366-6964.2015.07.025

Abstract

Abstract:

This experiment was conducted to screen the transcription factor binding sites of MyoDI promoter in Guizhou Guanling cattle．According to the sequence of bovine MyoDI gene published in GenBank，the specific primer was designed to amplify the promoter region of MyoDI gene in Guanling cattle．The PCR products was used to construct the recombinant cloning vector pUCM-T-MyoDI-pro．Positive plasmids were authenticated by sequencing.Then screening experiment and bioinformatics analysis were applied to screen the transcription factor binding sites of MyoDI promoter in Guanling cattle．The results showed that the transcription factor binding sites of MyoDI promoter in Guizhou Guanling cattle had SATB1，Xbp，MEF2，Pax-3，Pbx1，PPAR，TFⅡD，COUP-TF，Gfi-1，HNF-1，HOX4C，NRF2(ARE)，MEF1，RXR，SMUC，Snail，MyoD and VDR．Combined with online software analysis and literature，we ultimately screened out the MyoD，TFIID，Pax3，MEF1，VDR and MEF2 in MyoDI promoter.These transcription factors play an important role in regulation of promoter activity．The result indicate that the Guanling cattle MyoDI promoter contain MyoD，TFIID，Pax3，MEF1，VDR and MEF2 transcription factor binding sites.

CLC Number:

S823.2

ZHANG Wen，XU Hou-qiang，CHEN Wei，CHEN Xiang，HUAN Cong-cong，XIA Dan，ZHOU Di. The Screening of Transcription Factor Binding Sites of MyoDI Promoter in Guanling Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1259-1267.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.07.025

https://www.xmsyxb.com/EN/Y2015/V46/I7/1259

References

［1］ALVES H J，ALVARES L E，GABRIEL J E.Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos［J］.Brazilian J Med Biol Res，2003，36(2)：191-197.
［2］田璐，李俊雅，许尚忠，等.动物生肌决定因子的研究进展［J］.黄牛杂志，2005，(1)：43-46.
TIAN L，LI J Y，XU S Z，et al.Research progress of animal Myostatin gene［J］.Journal of Yellow Cattle Science，2005，(1)：43-46.(in Chinese)
［3］邱海芳，汪超，张庆德，等.猪MyoDl基因的多态性检测及与肉质性状的关联分析［J］.华中农业大学学报，2010，29(5)：593-596．
QIU H F，WANG C，ZHANG Q D，et al．PCR-RFLP polymorphism detection and relationship with meat quality traits of MyoD1 gene in pig［J］.Journal of Huazhong Agricultural University，2010，29(5)：593-596．(in Chinese)
［4］田璐，许尚忠，岳文斌，等.MyoD基因对肉牛胴体性状影响的分析［J］.遗传，2007，29(3)：313-318.
TIAN L，XU S Z，YUE W B，et al．Relationship between genotypes at MyoD locus and carcass traits in cattle［J］.Hereditas，2007，29(3)：313-318．(in Chinese)
［5］李永平，梁炳生.MyoD肌形成作用机制研究进展［J］.国际骨科学杂志，2007，28(1)：37-40.
LI Y P，LIANG B S.Progress of muscle formation mechanism of MyoD［J］.International Journal of Orthopaedics，2007，28(1)：37-40.(in Chinese)
［6］李飞，许厚强，陈伟，等.关岭牛MyoDⅠ基因启动子报告质粒的构建及活性验证［J］.基因组学与应用生物学，2013，(4)：459-466.
LI F，XU H Q，CHEN W，et al.Verification of activity and construction of the report plasmid of MyoDⅠgene promoter in Guanling cattle［J］.Genomics and Applied Biology，2013，(4)：459-466.(in Chinese)
［7］KNE?EVIC＇ D，-DURKIN I，KUŠEC G，et al.Influence of C489T SNP at MYOD1 gene on carcass，meat quality traits and chemical composition of hybrid pigs［J］.ACS，2013，78(3)：193-196.
［8］李硕，郝斐，梁浩，等.牛肌细胞生成素基因(MyoG)启动子的活性及组织特异性分析［J］.农业生物技术学报，2014，22(1)：87-93.
LI S，HAO F，LIANG H，et al.Activity and tissue- specific analysis of bovine(Bos) Myogenin gene (MyoG) promoter［J］.Journal of Agricultural Biotechnology，2014，22(1)：87-93.(in Chinese)
［9］GALICIA V G，DI M S，LIAN X J，et al.Regulation of eukaryotic initiation factor 4AII by MyoD during murine Myogenic cell differentiation［J］.PLoS ONE，2014，9(1)：e87237.
［10］罗启华，王安娜.贵州关岭黄牛发展现状与思考［J］.草食家畜，2011，(4)：15-16.
LUO Q H，WANG A N.Guizhou Guanling cattle development status and thinking［J］.Gress-feeding Livestock，2011，(4)：15-16.(in Chinese)
［11］闫晓红，王宁.转录因子与microRNA在基因表达调控中的功能联系及差异［J］.中国生物化学与分子生物学报，2010，10：892-897.
YAN X H，WANG N．Connections and differences between gene expression regulated by transcription factors and MicroRNAs［J］.Chinese Journal of Biochemistry and Molecular Biology，2010，10：892-897.(in Chinese)
［12］张娜，尹继刚，孙高超，等.真核生物转录因子及其研究方法进展［J］.动物医学进展，2009，(1)：75-79.
ZHANG N，YIN J G，SUN G C，et al．Progress on transcription factors and theirs methods of study in eukaryon［J］.Progress in Veterinary Medicine，2009，(1)：75-79.(in Chinese)
［13］赵丹丹，刘聪聪，贾明玉，等.肌肉特异性基因启动子的上游转录调控元件研究进展［J］.中国细胞生物学学报，2012，(5)：103-108.
ZHAO D D，LIU C C，JIA M Y，et al．Progress of upstream transcriptional control elements in promoters of skeletal specific genes［J］.Chinese Journal of Cell Biology，2012，(5)：103-108.(in Chinese)
［14］王秋华，曹允考，李树峰，等.牛MyoG 基因启动子的克隆及功能的初步分析［J］.畜牧兽医学报，2012，43(1)：37-43.
WANG Q H，CAO Y K，LI S F，et al.Cloning and preliminary functional analysis of bovine MyoG promoter［J］.Acta Veterinaria et Zootechnica Sinica，2012，43(1)：37-43.(in Chinese)
［15］BRYANTSEV A L，BAKER P W，LOVATO T L.Differential requirements for Myocyte Enhancer Factor-2 during adult MyoGenesis in Drosophila［J］.Dev Biol，2012，361(2)：191-207.
［16］DODOU E，XU S M，BLACK B L.MEF2c is activated directly by MyoGenic basic helix-loop- helixproteins during skeletal muscle development in vivo［J］.Mech Dev，2003，120(9)：1021-1032.
［17］HENNEBRY A，BERRY C.SIRIETT V.Myostatin regμlates fiber-type composition of skeletal muscle by regμlating MEF2 and MyoD gene expression［J］.Am J Physiol Cell Physiol，2009，296 (3)：C525-C534.
［18］JUNG S Y，KO Y G.TRIM72，a novel negative feedback regμlator of MyoGenesis，is transcriptionally activated by the synergism of MyoD (or MyoGenin) and MEF2［J］.Biochem Biophys Res Commun，2010，396(2)：238-245.
［19］WEINTRAUB H，DAVIS R，LOCKSHON D，et al.MyoD binds cooperatively to two sites in a targetenhancer sequence：Occupancy of two sites is required foractivation［J］.Proc Natl Acad Sci USA，1990；87(15)：5623-5627.
［20］KNOLL A，NEBOLA M，DVORKA J，et al.Detection of a DdeⅠPCR-RFLP within intron 1 of the porcine (MYF3) locus［J］.Anim Genet，1997，28(4)：308-322.
［21］POTTHOFF M J，OLSON E N.MEF2：A central regulator of diverse developmental programs［J］.Development，2007；134(23)：4131-4140.
［22］CUNHA P M，SANDMANN T，GUSTAFSON E H，et al.Combinatorial binding leads to diverse regula-tory responses：Lmd is a tissue-specific modulator of Mef2 activity［J］.PLoS Genet，2010，6(7)：e1001014.
［23］GOULDING M，LUMSDEN A，PAQUETTE A J.Regulation of Pax-3 expression in the dermomyotome and its role in muscle development［J］.Development，1994，120：957-971.
［24］TAJBAKHSH S，ROEANEOURT D，COSSU G，et al.Redefining the genetic hierarchies controlling skeletal myogenesis：Pax-3 and Myf-5 act upstream of MyoD［J］.Cell，1997，89：127-138.
［25］RELAIX F，ROCANCOURT D，MANSOURI A，et al．Pax3/Pax7-dependent population of skeletal muscle progenitor eels［J］.Nature，2005，435(7044)：948-953．
［26］BUCKINGHAM M，RELAIX F.The role of Pax genes in the develop-ment of tissues and organs：Pax3 and Pax7 regulate muscle pro-genitor cell functions［J］.Annu Rev Cell Dev Biol，2007，23：645-673.
［27］KUANG S，CHARGE S B，SEALE P，et al.Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis［J］.J Cell Biol，2006，172(1)：103-113.
［28］HU P，GELES K G，PAIK J H，et al.Co-dependent activators direct myoblast specific MyoD transcription［J］.Cell，2008，15(4)：534-546．
［29］吴俊静，梅书棋，彭先文，等.肉质相关基因TCAP启动子与转录因子MyoD结合的ChIP分析［J］．湖北农业科学，2013，22：5612-5614，5617．
WU J J，MEI S Q，PENG X W，et al.Detection of MyoD transcription factor binding with TCAP gene promoter by chromatin immunoprecipitation［J］．Hubei Agricultural Sciences，2013，22：5612-5614，5617．(in Chinese)

[1]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[2]	NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.
[3]	WANG Wenxiang, DU Lili, HU Junwei, ZHANG Yanxiang, MA Minghao, DUAN Rui, QIAN Cong, WANG Xinyue, LI Sanlu, ZHANG Changqing, ZHANG Lupei, GAO Xue, XU Lingyang, LI Junya, GAO Huijiang. Mining Candidate Genes Related to Meat Quality Traits of Longissimus Lumborum in Pingliang Red Steer Based on Transcriptome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4589-4604.
[4]	MA Haoran, ZHANG Lupei, JIN Shengyun, BAO Jinshan, LI Hongyan, GAO Huijiang, XU Lingyang, WANG Zezhao, LI Junya. Assessment of the Genomic Relationships for Chinese Indigenous Beef Cattle Using High-density SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4174-4185.
[5]	ZHANG Junxing, ZHANG Hailiang, HAN Liyun, MA Yanfen, WEN Wan, ZHOU Jiamin, TIAN Jia, LU Tingting, MA Yun, WANG Yachun. Analysis on the Influencing Factors of Wellness Traits in Holstein Lactating Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2389-2401.
[6]	YANG Chuang, WU Longfei, LIU Guangbin, LI Yaokun, LIU Dewu, SUN Baoli. Expression Profile and Bioinformatics Analysis of lncRNA and Its Associated ceRNA Networks in Longissimus Dorsi from Lufeng Cattle and Leiqiong Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1951-1963.
[7]	GUO Mingpeng, MENG Yuan, WANG Honghao, WANG Yuan, CHE Leijie, SHEN Li, WANG Xi. Estimation of Genetic Parameters of Body Weight and Body Size Traits in Jinnan Cattle at Different Growth Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1452-1464.
[8]	YU Shiqiang, LI Liuxue, ZHAO Xiaobo, ZHAO Huiying, TU Yan, ZHAO Yuchao, JIANG Linshu. Differences and Correlations of Lactation Performance in Chinese Holstein Dairy Cows at Different Lactation Stages and Somatic Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1003-1014.
[9]	YANG Guang, XU Jing, LI Xin, HU Debao, GUO Yiwen, DING Xiangbin, GUO Hong, ZHANG Linlin. Effect of Interfering lncbMD on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1015-1025.
[10]	MA Zhijie, WANG Shikang, ZHANG Weizhong, GUO Weixing, ZHAO Haiming, LEI Chuzhao. Y-chromosome Genomic Diversity and Paternal Origins of Qaidam Cattle and Mongolian Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1026-1033.
[11]	NIU Ruili, SONG Hanan, WU Yue, WANG Yunan, GAO Yefan, GUAN Weijun, ZONG Xianchun. Isolation, Culture and Biological Characteristics of Epidermal Stem Cells from Simmental Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 168-177.
[12]	LI Hongwei, XU Lingyang, WANG Zezhao, CAI Wentao, ZHU Bo, CHEN Yan, GAO Xue, ZHANG Lupei, GAO Huijiang, LI Junya. Genome-wide Association Study of Slaughter Traits Based on Haplotype in Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4232-4243.
[13]	CHANG Yao, SU Guosheng, LI Yanhua, LI Xiang, MA Zhu, WANG Yachun. Estimating Genetic Parameters for Body Weights using Pedigree and Genotype-pedigree based Approaches in Holstein Heifers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3759-3768.
[14]	XU Jingyi, XU Haoqi, HU Lirong, ZHANG Fan, GAO Qing, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, GUO Gang, WANG Yachun. Association Analysis of MET Gene Single Nucleotide Polymorphism with Reproduction and Milk Production Traits in Chinese Holstein Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3769-3785.
[15]	WANG Chuanchuan, MU Tong, FENG Xiaofang, YU Baojun, ZHANG Juan, GU Yaling. Identification of Key Candidate Genes for Milk Fat Metabolism in Dairy Cows Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3421-3433.

The Screening of Transcription Factor Binding Sites of MyoDI Promoter in Guanling Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments